Antimicrobial effect in vitro of five soft lenses multipurpose solutions against Candida albicans y Aspergillus fumigatus

Abstract

Colonization by fungi in the soft contact lenses can cause severe ocular infections that can be prevented with solutions multipurpose. <em><strong>Objective:</strong></em> to evaluate the antimicrobial effectiveness in vitro of five commercial solutions multipurpose against two fungi, Candida albicans (leavening) and Aspergillus fumigatus (mould). Materials and <em><strong>Methods:</strong></em> five commercial solutions multipurpose, Solution 1 (PHMB to 0.0001%), Solution 2 (Polyquad to 0.001%), Solution 3 (PAPB), Solution 4 (Trimetoprim to 0.01%), Solution 5 (hydrogen Peroxide to 3%), were analyzed in vitro as opposed to Candida albicans and Aspergillus fumigatus. The inocule was standardized using three concentrations for each microoganism. The effectiveness of the solutions was determined by calculation of the percentage average of reduction of training units of colonies obtained to time zero of disinfection and the time recommended by each commercial house (4 or 6 hours). Each test was made by triplicate. All the tests were made parallels with a growth control. The inocule without multipurpose solution and a positive control of the 100% of elimination of the microorganisms with hydrogenate peroxide to 3.7%. <em><strong>Results:</strong></em> For C. albicans: Solutions 1, 2, 3 and 5 showed 100% of elimination at time 0 and at the time suggested by the manufacturer (4 or 6 hours); solution 4, to the 6 hours of disinfection (according fumigatusto the recommendation by the commercial house), presented: with the lowest concentration of C albicans, a 85% of elimination, with the second concentration a 31% of elimination and in the highest concentration of the microorganism, obtained a negative result, -74%. For A. fumigatus: solution 5 demonstrated a 100% of elimination at time 0 and at 6 hours of disinfection to the three concentrations; of the other four, to the time recommended by the commercial house (4 or 6 hours) the most effective solution was the 2, with a 91.33% in average with the three concentrations of Aspergillus, followed of the solution 1 (84.6%), solution 3 (71.6%) and solution 4 with the smaller percentage average of elimination 38.66%. Conclusions: Among the five solutions multipurpose evaluated, the Solution 4 demonstrated less effectiveness. Solutions 1 and 2 demonstrated cidal effect for C albicans, and fungistatic effect against A. fumigatus. The solution 3 presented effectiveness of the 100% for C. albicans, and for A. fumigatus its effect was microbiostatic. Solution 5 demonstrated fungicidal effect against both fungi evaluated. The Aspergillus fumigatus offered greater resistance to the solutions that the C. albicans, except for the solution 5 with active principle hydrogen peroxide.